Stable Cell Line Development

Expicho s cells are transfected cloned and selected in expicho expression medium followed by the scale up in the bioproduction amenable expicho stable production medium.

Stable cell line development.

Virtually any cdna mab and multigenic applications can be packaged into the gpex retrovector and used to insert your gene of interest into cells. The development of high yield production cell lines starts with the cloning of the gene of interest into sure tech vectors that bear selexis sge selexis genetic elements. There are two types of stable cell lines. These optimally designed dna expression vectors are then transfected into the fully documented selexis sure cho m cell line using the sure fection procedure.

This protocol describes the procedure for creating a stable cell line based on the expicho expression system for use in commercial bioproduction. This is a very common problem for many stable cell lines. Stable expression of target gene in cell lines overcomes the low transfection efficiency of transient expression and produces more target proteins. That s why gibco products are designed to provide you with integrated stable cell line development solutions to simplify your development workflow and accelerate your processes to commercial manufacture.

However not all stable cell lines are equally stable. You want to develop a stable cell line because it is stable in expressing the target protein. Stable cell line generation. Typical cell line workflow step 1.

Traceability of cell history and cell line generation process high productivity and robust stable cell line development platforms. The jump in technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using phic31 integrase. Stable cell line development cell engineering group offers services for cell line development from dna sequences to rcb stability assessment. Proteogenix strives to overcome the economic issues related to stable cell line generation by offering the most productive ip free cell lines on the market.

We understand that cell line development involves multiple complex processes requiring consistency and reproducible results. Gpex cell line development technology is a proven technology that generates high performance highly stable production cell lines. After several passages some stable cell lines may become unstable with their expression diminishing slowly. Foreign dna encoding the protein of interest often includes an additional selectable marker.

By applying single cell clonings from the selected stable pools selexis identifies high expressing clonal cell lines that are then evaluated for their. To date over 600 different mab and mab fusions and over 80 different recombinant proteins have been produced using the gpex system with 120 ongoing clinical trials utilizing therapeutic candidates developed.